At the interface of proteins and glycans, the imaginary STADLMANN lab aims at developing analytical technologies for polypeptide-linked carbohydrates.
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selected publications
Capraz T, Kienzl NF, Laurent E, Perthold JW, Föderl-Höbenreich E, Grünwald-Gruber C, Maresch D, Monteil V, Niederhöfer J, Wirnsberger G, Mirazimi A, Zatloukal K, Mach L, Penninger JM, Oostenbrink C, Stadlmann J. Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptor. Elife. 2021 Dec 20;10:e73641. doi: 10.7554/eLife.73641. PMID: 34927585; PMCID: PMC8730730.
Raglow Z, McKenna MK, Bonifant CL, Wang W, Pasca di Magliano M, Stadlmann J, Penninger JM, Cummings RD, Brenner MK, Markovitz DM. Targeting glycans for CAR therapy: The advent of sweet CARs. Mol Ther. 2022 Sep 7;30(9):2881-2890. doi: 10.1016/j.ymthe.2022.07.006. Epub 2022 Jul 12. PMID: 35821636; PMCID: PMC9481985.
Ctortecka C, Krššáková G, Stejskal K, Penninger JM, Mendjan S, Mechtler K, Stadlmann J. Comparative Proteome Signatures of Trace Samples by Multiplexed Data-Independent Acquisition. Mol Cell Proteomics. 2022 Jan;21(1):100177. doi: 10.1016/j.mcpro.2021.100177. Epub 2021 Nov 15. PMID: 34793982; PMCID: PMC8717550.
Kawahara R, Chernykh A, Alagesan K, Bern M, Cao W, Chalkley RJ, Cheng K, Choo MS, Edwards N, Goldman R, Hoffmann M, Hu Y, Huang Y, Kim JY, Kletter D, Liquet B, Liu M, Mechref Y, Meng B, Neelamegham S, Nguyen-Khuong T, Nilsson J, Pap A, Park GW, Parker BL, Pegg CL, Penninger JM, Phung TK, Pioch M, Rapp E, Sakalli E, Sanda M, Schulz BL, Scott NE, Sofronov G, Stadlmann J, Vakhrushev SY, Woo CM, Wu HY, Yang P, Ying W, Zhang H, Zhang Y, Zhao J, Zaia J, Haslam SM, Palmisano G, Yoo JS, Larson G, Khoo KH, Medzihradszky KF, Kolarich D, Packer NH, Thaysen-Andersen M. Community evaluation of glycoproteomics informatics solutions reveals high-performance search strategies for serum glycopeptide analysis. Nat Methods. 2021 Nov;18(11):1304-1316. doi: 10.1038/s41592-021-01309-x. Epub 2021 Nov 1. Erratum in: Nat Methods. 2021 Dec 10;: Erratum in: Nat Med. 2022 Jan;28(1):214. PMID: 34725484; PMCID: PMC8566223.
Stadlmann J., Taubenschmid J., et al. Comparative glycoproteomics of stem cells identifies new players in ricin toxicity, Nature (2017).
Stadlmann J., et al. Analysis of PNGase F-Resistant N-Glycopeptides Using SugarQb for Proteome Discoverer 2.1 Reveals Cryptic Substrate Specificities, Proteomics (2018).
Beveridge R, Stadlmann J, Penninger JM, Mechtler K. A synthetic peptide library for benchmarking crosslinking-mass spectrometry search engines for proteins and protein complexes. Nat Commun. 2020 Feb 6;11(1):742. DOI: 10.1038/s41467-020-14608-2
Stadlmann J, Hudecz O, Krššáková G, Ctortecka C, Van Raemdonck G, Op De Beeck J, Desmet G, Penninger JM, Jacobs P, Mechtler K. Improved Sensitivity in Low-Input Proteomics Using Micropillar Array-Based Chromatography. Anal Chem. 2019 Nov 19;91(22):14203-14207. DOI: 10.1021/acs.analchem.9b02899
Wirnsberger G, Zwolanek F, Stadlmann J, Tortola L, Liu SW, Perlot T, Järvinen P, Dürnberger G, Kozieradzki I, Sarao R, De Martino A, Boztug K, Mechtler K, Kuchler K, Klein C, Elling U, Penninger JM. Jagunal homolog 1 is a critical regulator of neutrophil function in fungal host defense. Nat Genet. 2014 Sep;46(9):1028-33. DOI: 10.1038/ng.3070
Hagelkruys A, Wirnsberger G, Stadlmann J, Woehner M, Horrer M, Vilagos B, Jonsson G, Kogler M, Tortola L, Novatchkova M, Boenelt P, Hoffmann D, Koglgruber R, Steffen U, Schett G, Busslinger M, Bergthaler A, Klein C, Penninger JM. A crucial role for Jagunal homolog 1 in humoral immunity and antibody glycosylation in mice and humans. J Exp Med. 2021 Jan;218(1) . doi:10.1084/jem.20200559.
Dorfer V, Pichler P, Stranzl T, Stadlmann J, Taus T, Winkler S, Mechtler K. MS Amanda, a universal identification algorithm optimized for high accuracy tandem mass spectra. J Proteome Res. 2014 Aug 1;13(8):3679-84. doi: 10.1021/pr500202e
Stadlmann J, Pabst M, Altmann F. Analytical and Functional Aspects of Antibody Sialylation. J Clin Immunol. 2010 Apr 14. DOI: 10.1007/s10875-010-9409-2
Stadlmann J, Weber A, Pabst M, Anderle H, Kunert R, Ehrlich HJ, Peter Schwarz H, Altmann F. A close look at human IgG sialylation and subclass distribution after lectin fractionation. Proteomics. 2009 Sep;9(17):4143-53. DOI: 10.1002/pmic.200800931